Table 2

Extracellular vesicle characterisation as reported in included papers

First authorYearData shown in paperSize/morphologyMembrane-associated markersCytosolic proteins recovered in EVs
Nanoparticle trackingMicroscopyCD63CD81CD9CD31CD144CD45CD29CD41bMCSPTSG101AlixHSP70Annexin V
Chen 272016x**
Doeppner 282015x**
Geng292019x
Han302018x**
Huang312018✓ TEMWBWBWB
Jiang322018✓ TEMWBWBWBWB
Kalani182016WB
Lee332016x
Liu342019✓ cryo-EMWBWBWB
Moon352019✓ TEM
Otero-Ortega362017✓ EMIFWB
Otero-Ortega372018✓ EMIFWB
Pan382016FCFCFC
Shan192013✓ cryo-EMFCFCFC
Webb162018✓ EM
Webb172018✓ SLIMFCFCFC
Xin392013x
Xin402017a✓ TEMWBWB
Xin412017bx***
Zheng152019DLS✓ TEMWBWBWBWB
  • First International Society for Extracellular Vesicles (ISEV) guidelines on EV characterisation and reporting released in 2014, updated in 2018.

  • *Denotes characterisation reported but not presented.

  • DLS, dynamic light scattering; EM, electron microscopy; EV, extracellular vesicle; FC, flow cytometry; IF, immunofluorescence; SLIM, spatial light interference microscopy; TEM, transmission electron microscopy; WB, western blot.